Noninvasive penetration resin materials repair early stage deciduous caries / 中国组织工程研究

10.3969/j.issn.2095-4344.2013.25.025

Simultaneous determination of gestodene, etonogestrel and ethinylestradiol in plasma by LC-MS/MS following derivatization / 药学学报

To establish a sensitive and specific method for simultaneous determination of gestodene, etonogestrel and ethinylestradiol in plasma by LC-MS/MS, plasma samples were extracted and derivatized before injection. An ESI ion source was used and operated in the positive ion mode with multiple reaction monitoring (MRM). Norgestrel was chosen as internal standard and performed on a C18 (100 mm x 2.1 mm, 5 microm) column. The concentrations of gestodene, etonogestrel and ethinylestradiol were measured, using step-gradient mobile phase and step-gradient flow rate. The method was validated over the concentration range of 0.1-20 ng x mL(-1) for gestodene and etonogestrel and 0.01-2 ng x mL(-1) for ethinylestradiol, and showed excellent linearity. The intra- and inter-assay accuracy and precision were below 10.0% and recovery was 93.6%-110.9% over the three concentration levels evaluated. The method was applied in pharmacokinetic study of the compound gestodene patch and the compound etonogestrel patch in rabbits. The LC-MS/MS method was selective, accurate and sensitive, especially the LOQ were 100 pg x mL(-1) for gestodene and etonogestrel and 10 pg x mL(-1) for ethinylestradiol. The method was successfully applied in pharmacokinetic study for contraceptives.

Prosthetic Reconstruction for Bladder with Glutaraldehyde-Stabilized Aminia in Rabbits / 中华创伤杂志

The result of experimental study of the bladder replacement with the glutaraldehyde-stalized amimia in 10 rabbits is reported. This kind of aminia, as a substitute for the bladder, replaced 1/3 bladder (n=4), 2/3 (n=5) and subtotal bladder (n=1) in rabbits, with observation for 2 weeks (n=4), 5 weeks (n=5), 6 months (n=1) and 30 months (n=1). The result showed that all replacements of the bladders were succesful. The aminia is probably the best biologic material at present for replacement of the bladder

Dynamic change of plasma-angiotensin Ⅱ level in crush syndrome and its significance / 中国病理生理杂志

This paper reports an experimental study of the dynamic change of plasma-angiotensin Ⅱ (AgⅢ) level and its significance in 24 rabbits with crush syndrome. The average values of Ag Ⅱ at 24, 48 and 72 hours after the crush experiment were about 5.5 times as before, the increment of Ag Ⅱ at 24 and 48 hours after the crush were significant (P